In vitro cultivation of Leishmania donovani promastigotes: Growth potential of human urine as replacement of fetal calf serum
نویسندگان
چکیده
In vitro cultivation of Leishmania parasites plays an important role in diagnosis and treatment leishmaniasis vaccine drug development studies. There is no information about the effects urine within culture on infectivity parasites. In this study we used two media with different overlays to enhance growth rates donovani.
منابع مشابه
Use of chicken serum as a good replacement for the fetal calf serum in cultivation of promastigotes of Leishmania major
Genus Leishmania is a protozoan parasite that causes different severe diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, for mass cultivation and the most expensive ingredient in these media. In the present work, the efficacy of chicken serum was evaluated in Leishmania culture media. The results indicated that, the (10%) chicken serum enriched culture medium su...
متن کاملEvaluating the Coconut Water as a Replacement for the Fetal Calf Serum in Cultivation of Promastigotes of Leishmania Infantum
Backgrounds and Aims: The protozoan parasites of the genus Leishmania can be stated as the causative agents of the various clinical diseases. Fetal Calf Serum (FCS) is the major part of the Leishmania culture media, which is the most expensive ingredient in these media. The coconut water is packed with nutrients that yield an array of health benefits. Materials and Methods: In the present st...
متن کاملuse of chicken serum as a good replacement for the fetal calf serum in cultivation of promastigotes of leishmania major
genus leishmania is a protozoan parasite that causes different severe diseases. fetal calf serum (fcs) is the major part of the leishmania culture media, for mass cultivation and the most expensive ingredient in these media. in the present work, the efficacy of chicken serum was evaluated in leishmania culture media. the results indicated that, the (10%) chicken serum enriched culture medium su...
متن کاملevaluating the coconut water as a replacement for the fetal calf serum in cultivation of promastigotes of leishmania infantum
backgrounds and aims: the protozoan parasites of the genus leishmania can be stated as the causative agents of the various clinical diseases. fetal calf serum (fcs) is the major part of the leishmania culture media, which is the most expensive ingredient in these media. the coconut water is packed with nutrients that yield an array of health benefits. materials and methods: in the present study...
متن کاملSurface antigens of Leishmania donovani promastigotes
Surface antigen profiles of Leishmania donovani promastigote isolates have been studied. Surface patterns of Brazilian and African isolates display remarkable similarities and are extremely simple, consisting of three major peptides of 65,000, 25,000, and 23,000 mol wt. Surface iodination and biosynthetic labeling coupled to immunoprecipitation techniques revealed that a single major determinan...
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ژورنال
عنوان ژورنال: Annals of systems biology
سال: 2021
ISSN: ['2692-4765']
DOI: https://doi.org/10.17352/asb.000010